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aec substrate buffer  (Millipore)


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    Structured Review

    Millipore aec substrate buffer
    Aec Substrate Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/substrate+buffer+aec/aec+substrate+buffer/pmc09696641-50-39-42
    Average 90 stars, based on 1 article reviews
    aec substrate buffer - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: Dynamics of humoral and cellular immune responses after homologous and heterologous SARS-CoV-2 vaccination with ChAdOx1 nCoV-19 and BNT162b2
    Article Snippet: .. Plates were washed with PBS containing 0.05% Tween-20 (Sigma-Aldrich) and incubated with biotinylated anti-human IFN-γ monoclonal antibody (clone 7-B6-1, Mabtech) at 0.2 μg/well for 2 h. Following a second wash step with PBS containing 0.05% Tween-20, plates were incubated with an avidin-biotinylated peroxidase complex (VECTASTAIN ® Elite ABC-HRP Kit, Vector Laboratories) for 1-2 h. After final washing steps with first PBS containing 0.05% Tween-20 and then PBS, plates were developed by the addition of AEC substrate solution (Sigma-Aldrich) for 15 minutes. .. Subsequently, plates were washed with water, dried for 24 h in the dark, and analyzed on an ImmunoSpot ® Analyzer (Cellular Technologies Limited).

    Article Title: Plasmablast, Memory B Cell, CD4+ T Cell, and Circulating Follicular Helper T Cell Responses to a Non-Replicating Modified Vaccinia Ankara Vaccine
    Article Snippet: Plates were washed four times in PBST and incubated for 1h at room temperature with streptavidin-HRP (Vector Laboratories #A 2004) diluted 1:1000 in PBST with 1% FBS. .. Plates were washed three times each in PBST, then with PBS, followed with incubation with 3-Amino-9-ethylcarbazole (AEC) substrate kit (EMD Millipore Corporation, Substrate #152226, Buffer #152224) for 10 min until spot development. .. Plates were washed with water and allowed to dry; images were obtained using a CTL ELISpot plate reader.

    Article Title: Dynamics of humoral and cellular immune responses after homologous and heterologous SARS-CoV-2 vaccination with ChAdOx1 nCoV-19 and BNT162b2.
    Article Snippet: .. Plates were washed with PBS containing 0.05% Tween-20 (Sigma-Aldrich) and incubated with biotinylated anti-human IFN-g monoclonal antibody (clone 7-B6-1, Mabtech, RRID:AB_907272) at 0.2 μg/well for 2 h. Following a second wash step with PBS containing 0.05% Tween-20, plates were incubated with an avidin-biotinylated peroxidase complex (VECTASTAIN Elite ABC-HRP Kit, Vector Laboratories) for 1-2 h. After final washing steps with first PBS containing 0.05% Tween-20 and then PBS, plates were developed by the addition of AEC substrate solution (Sigma-Aldrich) for 15 minutes. .. Subsequently, plates were washed with water, dried for 24 h in the dark, and analyzed on an ImmunoSpot Analyzer (Cellular Technologies Limited).

    Article Title: Dynamics of humoral and cellular immune responses after homologous and heterologous SARS-CoV-2 vaccination with ChAdOx1 nCoV-19 and BNT162b2
    Article Snippet: .. Plates were washed with PBS containing 0.05% Tween-20 (Sigma-Aldrich) and incubated with biotinylated anti-human IFN-γ monoclonal antibody (clone 7-B6-1, Mabtech, RRID:AB_907272) at 0.2 μg/well for 2 h. Following a second wash step with PBS containing 0.05% Tween-20, plates were incubated with an avidin-biotinylated peroxidase complex (VECTASTAIN® Elite ABC-HRP Kit, Vector Laboratories) for 1-2 h. After final washing steps with first PBS containing 0.05% Tween-20 and then PBS, plates were developed by the addition of AEC substrate solution (Sigma-Aldrich) for 15 minutes. .. Subsequently, plates were washed with water, dried for 24 h in the dark, and analyzed on an ImmunoSpot® Analyzer (Cellular Technologies Limited).

    Article Title: Evaluation of the Delivery of a Live Attenuated Porcine Reproductive and Respiratory Syndrome Virus as a Unit Solid Dose Injectable Vaccine
    Article Snippet: .. Following three washes in PBSt, polyclonal goat anti-mouse immunoglobulin-horseradish peroxidase (HRP) conjugate (Thermo Fisher Scientific), diluted 1:4000 in PBSt, was added and incubated at room temperature for 60 min. After washing three times in PBSt, AEC (3-amino-9-ethylcarbazole) prepared in AEC substrate buffer (Sigma-Aldrich) was added, and the cells were incubated for 20 min at room temperature. ..

    Avidin-Biotin Assay:

    Article Title: Dynamics of humoral and cellular immune responses after homologous and heterologous SARS-CoV-2 vaccination with ChAdOx1 nCoV-19 and BNT162b2
    Article Snippet: .. Plates were washed with PBS containing 0.05% Tween-20 (Sigma-Aldrich) and incubated with biotinylated anti-human IFN-γ monoclonal antibody (clone 7-B6-1, Mabtech) at 0.2 μg/well for 2 h. Following a second wash step with PBS containing 0.05% Tween-20, plates were incubated with an avidin-biotinylated peroxidase complex (VECTASTAIN ® Elite ABC-HRP Kit, Vector Laboratories) for 1-2 h. After final washing steps with first PBS containing 0.05% Tween-20 and then PBS, plates were developed by the addition of AEC substrate solution (Sigma-Aldrich) for 15 minutes. .. Subsequently, plates were washed with water, dried for 24 h in the dark, and analyzed on an ImmunoSpot ® Analyzer (Cellular Technologies Limited).

    Article Title: Dynamics of humoral and cellular immune responses after homologous and heterologous SARS-CoV-2 vaccination with ChAdOx1 nCoV-19 and BNT162b2.
    Article Snippet: .. Plates were washed with PBS containing 0.05% Tween-20 (Sigma-Aldrich) and incubated with biotinylated anti-human IFN-g monoclonal antibody (clone 7-B6-1, Mabtech, RRID:AB_907272) at 0.2 μg/well for 2 h. Following a second wash step with PBS containing 0.05% Tween-20, plates were incubated with an avidin-biotinylated peroxidase complex (VECTASTAIN Elite ABC-HRP Kit, Vector Laboratories) for 1-2 h. After final washing steps with first PBS containing 0.05% Tween-20 and then PBS, plates were developed by the addition of AEC substrate solution (Sigma-Aldrich) for 15 minutes. .. Subsequently, plates were washed with water, dried for 24 h in the dark, and analyzed on an ImmunoSpot Analyzer (Cellular Technologies Limited).

    Article Title: Dynamics of humoral and cellular immune responses after homologous and heterologous SARS-CoV-2 vaccination with ChAdOx1 nCoV-19 and BNT162b2
    Article Snippet: .. Plates were washed with PBS containing 0.05% Tween-20 (Sigma-Aldrich) and incubated with biotinylated anti-human IFN-γ monoclonal antibody (clone 7-B6-1, Mabtech, RRID:AB_907272) at 0.2 μg/well for 2 h. Following a second wash step with PBS containing 0.05% Tween-20, plates were incubated with an avidin-biotinylated peroxidase complex (VECTASTAIN® Elite ABC-HRP Kit, Vector Laboratories) for 1-2 h. After final washing steps with first PBS containing 0.05% Tween-20 and then PBS, plates were developed by the addition of AEC substrate solution (Sigma-Aldrich) for 15 minutes. .. Subsequently, plates were washed with water, dried for 24 h in the dark, and analyzed on an ImmunoSpot® Analyzer (Cellular Technologies Limited).

    Staining:

    Article Title: Type I IFN blockade uncouples immunotherapy-induced antitumor immunity and autoimmune toxicity
    Article Snippet: Color was developed using sequential treatment with the VECTASTAIN ABC Kit (Vector Laboratories) and the ImmPACT AMEC Red Peroxidase Substrate Kit (Vector Laboratories), followed by hematoxylin counterstaining. .. CD8a mAb (53-6.7, BD Biosciences; 1:50 dilution) staining was performed on sections from tissues snap-frozen in Tissue-Tek OCT (Sakura), fixed with acetone and ethanol, and treated with 3% hydrogen peroxide before development using a Rat-on-Mouse HRP-Polymer Kit (Biocare Medical) and an AEC Chromogen Substrate Solution Kit (Sigma-Aldrich). .. MHC I mAb (27-11-13, Abcam) was diluted 1:100 in Powervision IHC Super Blocker (Leica) and preabsorbed with AffiniPure Fab Fragment Rabbit Anti-Mouse IgG and mouse serum (Jackson ImmunoResearch) before staining of frozen tissue sections on the Bond RX Automated Stainer (Leica) using a Bond Polymer Refine Red Detection System (Leica).

    other:

    Article Title: Nickel, palladium and rhodium induced IFN-gamma and IL-10 production as assessed by in vitro ELISpot-analysis in contact dermatitis patients
    Article Snippet: Subsequent to specific substrate addition, the red spots were developed using AEC-chromogen solution (Sigma, St Louis, MO, USA) and analysed by the Automated ImmunoSpot Image Analyzer Software (AELVIS Tecnologies, TEMA ricerche, Italy).



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